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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha : |
10/02/2021 |
Actualizado : |
11/02/2021 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
BRITO, L.F; BERRY, D.; LARROQUE, H.; SCHENKEI, F.S.; CIAPPESONI, G.; O’BRIEN, A.; TORTEREAU, F.; UGARTE, E.; PALHIERE, I.; BAPST, B.; JAKOBSEN, J.; ANTONAKOS, G.; KOMINAKIS, A.; CLEMENT, V.; BRUNI, G.; LOYWYCK, V.; MASSENDER, E.; OLIVEIRA, H.R.; POSTA, J.; ASTRUC, J.M. |
Afiliación : |
L.F. BRITO, University of Guelph, Guelph, Canada; D. BERRY; H. LARROQUE, INRAE, Toulouse, France; F.S. SCHENKEL, University of Guelph, Guelph, Canada; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; A. O’BRIEN, TEAGASC, Fermoy, Ireland; F. TORTEREAU, INRAE, Toulouse, France; E. UGARTE, 5NEIKER, Vizcaya, Spain; I. PALHIERE, INRAE, Toulouse, France; B. BAPST, Qualitas AG, Zug, Switzerland; J. JAKOBSEN, NSG, Norway; G. ANTONAKOS, Frizarta, Agrinio, Greece; A. KOMINAKIS, Frizarta, Agrinio, Greece; V. CLEMENT, IDELE, Toulouse, France; G. BRUNI, ARAL, Crema, Italy; V. LOYWYCK, IDELE, Toulouse, France,; E. MASSENDER, University of Guelph, Guelph, Canada; H.R. OLIVEIRA, University of Guelph, Guelph, Canada; J. POSTA, ARAL, Crema, Italy; J.M. ASTRUC, IDELE, Toulouse, France. |
Título : |
Genetic evaluation systems and breeding programs in sheep and goats: an international perspective. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Annual Meeting of the European Federation of Animal Science, 17., No. 26, 2020. Virtual Meeting 1-4 December, 2020. DOI: DOI: 10.3920/978-90-8686-900-8. |
Páginas : |
p. 560 |
DOI : |
10.3920/978-90-8686-900-8. |
Idioma : |
Español |
Contenido : |
Genetic selection has been a key tool for improving productive performance in small ruminant populations around the world, especially in Europe. This has been achieved through selective breeding for numerous traits, using diverse genetic evaluation systems and breeding schemes. Knowledge of the alternative approaches taken is paramount to the design of efficient and integrated genomic breeding programs. In this study, we summarised information on 48 sheep and goat breeding programs, genetic and genomic evaluation systems and resources available in 12 countries involved in the SMARTER project. This was done using published reports and surveys distributed to all partners. Responses to the surveys reveals information for more than 9, 16, and 20 dairy goat, dairy sheep, and meat sheep breeds involved in genetic schemes, respectively, with ~3,083,562 animals included in data collection schemes. The main groups of traits recorded across countries are: (1) milk yield and composition, mastitis indicators, udder and
body conformation, and reproduction in dairy sheep and dairy goats; and (2) growth, reproduction, health, ultrasound, wool, and carcass in meat sheep. Seven countries have progeny testing schemes, but only 5 use artificial insemination. There are numerous challenges to be addressed (e.g. disparity of trait recording, SNP panels, statistical models used, joining pedigrees across countries as well as grouping breeds based on genetic similarity, and an average of ~30% of animals with unknown sires). However, there are many opportunities to use the current resources and develop collaborative approaches to optimise selection for novel breeding goals such as resilience and efficiency in small ruminants across countries. MenosGenetic selection has been a key tool for improving productive performance in small ruminant populations around the world, especially in Europe. This has been achieved through selective breeding for numerous traits, using diverse genetic evaluation systems and breeding schemes. Knowledge of the alternative approaches taken is paramount to the design of efficient and integrated genomic breeding programs. In this study, we summarised information on 48 sheep and goat breeding programs, genetic and genomic evaluation systems and resources available in 12 countries involved in the SMARTER project. This was done using published reports and surveys distributed to all partners. Responses to the surveys reveals information for more than 9, 16, and 20 dairy goat, dairy sheep, and meat sheep breeds involved in genetic schemes, respectively, with ~3,083,562 animals included in data collection schemes. The main groups of traits recorded across countries are: (1) milk yield and composition, mastitis indicators, udder and
body conformation, and reproduction in dairy sheep and dairy goats; and (2) growth, reproduction, health, ultrasound, wool, and carcass in meat sheep. Seven countries have progeny testing schemes, but only 5 use artificial insemination. There are numerous challenges to be addressed (e.g. disparity of trait recording, SNP panels, statistical models used, joining pedigrees across countries as well as grouping breeds based on genetic similarity, and an average of ~30% of ani... Presentar Todo |
Palabras claves : |
OVINOS; SHEEP. |
Asunto categoría : |
L01 Ganadería |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15000/1/Brito-et-al-EAAP2020.pdf
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Marc : |
LEADER 02889nam a2200385 a 4500 001 1061719 005 2021-02-11 008 2020 bl uuuu u01u1 u #d 024 7 $a10.3920/978-90-8686-900-8.$2DOI 100 1 $aBRITO, L.F 245 $aGenetic evaluation systems and breeding programs in sheep and goats$ban international perspective.$h[electronic resource] 260 $aAnnual Meeting of the European Federation of Animal Science, 17., No. 26, 2020. Virtual Meeting 1-4 December, 2020. DOI: DOI: 10.3920/978-90-8686-900-8.$c8686 300 $ap. 560 520 $aGenetic selection has been a key tool for improving productive performance in small ruminant populations around the world, especially in Europe. This has been achieved through selective breeding for numerous traits, using diverse genetic evaluation systems and breeding schemes. Knowledge of the alternative approaches taken is paramount to the design of efficient and integrated genomic breeding programs. In this study, we summarised information on 48 sheep and goat breeding programs, genetic and genomic evaluation systems and resources available in 12 countries involved in the SMARTER project. This was done using published reports and surveys distributed to all partners. Responses to the surveys reveals information for more than 9, 16, and 20 dairy goat, dairy sheep, and meat sheep breeds involved in genetic schemes, respectively, with ~3,083,562 animals included in data collection schemes. The main groups of traits recorded across countries are: (1) milk yield and composition, mastitis indicators, udder and body conformation, and reproduction in dairy sheep and dairy goats; and (2) growth, reproduction, health, ultrasound, wool, and carcass in meat sheep. Seven countries have progeny testing schemes, but only 5 use artificial insemination. There are numerous challenges to be addressed (e.g. disparity of trait recording, SNP panels, statistical models used, joining pedigrees across countries as well as grouping breeds based on genetic similarity, and an average of ~30% of animals with unknown sires). However, there are many opportunities to use the current resources and develop collaborative approaches to optimise selection for novel breeding goals such as resilience and efficiency in small ruminants across countries. 653 $aOVINOS 653 $aSHEEP 700 1 $aBERRY, D. 700 1 $aLARROQUE, H. 700 1 $aSCHENKEI, F.S. 700 1 $aCIAPPESONI, G. 700 1 $aO’BRIEN, A. 700 1 $aTORTEREAU, F. 700 1 $aUGARTE, E. 700 1 $aPALHIERE, I. 700 1 $aBAPST, B. 700 1 $aJAKOBSEN, J. 700 1 $aANTONAKOS, G. 700 1 $aKOMINAKIS, A. 700 1 $aCLEMENT, V. 700 1 $aBRUNI, G. 700 1 $aLOYWYCK, V. 700 1 $aMASSENDER, E. 700 1 $aOLIVEIRA, H.R. 700 1 $aPOSTA, J. 700 1 $aASTRUC, J.M.
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Registro original : |
INIA Tacuarembó (TBO) |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
24/10/2023 |
Actualizado : |
24/10/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
BRANDA-SICA, A.; NICOLINI, M.P.; ARTIGAS, R.; FEDERICI, M.; LLAMBÍ, S. |
Afiliación : |
ANDREA BRANDA SICA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA PAULA NICOLINI DUARTE, Universidad de la República, Centro Universitario de Tacuarembó, Instituto Superior de la Carne, Área Biología Molecular. Tacuarembó, Uruguay; RODY ARTIGAS, Universidad de la República, Facultad de Veterinaria, Unidad Académica de Genética y Mejora Animal. Montevideo, Uruguay; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA LLAMBÍ, Universidad de la República, Facultad de Veterinaria, Unidad Académica de Genética y Mejora Animal. Montevideo, Uruguay. |
Título : |
Optimización de PCR en tiempo real con curvas de disociación para la detección de la mutación causante de deficiencia de colesterol en bovinos Holando. [Optimization of Real-Time PCR-melting for detection of the Cholesterol-deficiency mutation in Holstein Friesian cattle]. |
Complemento del título : |
Sección Medicina Veterinaria. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Revista Cientifica Facultad de Ciencias Veterinarias, 2022, volume 32, e32137, 1-5. https://doi.org/10.52973/rcfcv-e32137 -- OPEN ACCESS. |
ISSN : |
0798-2259 (print); 2521-9715 (electronic). |
DOI : |
10.52973/rcfcv-e32137 |
Idioma : |
Inglés |
Notas : |
Article history: Received 15 March 2022, Accepted 30 March 2022, Published 21 June 2022. -- Correspondence author: Andrea Branda, email: abranda@inia.org.uy -- LICENSE: Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0. (https://creativecommons.org/licenses/by-nc-sa/4.0/ ). |
Contenido : |
RESUMEN: El objetivo de este estudio fue optimizar un análisis mediante PCR en tiempo real con curvas de disociación para la detección confiable y económica del inserto mutante de 7,5 Kb del elemento transponible bovino BoERVK en el exón 5 del gen de la Apolipoproteína B (APOB), determinante de la deficiencia de colesterol - CD - (OMIA 001965-9913). Asimismo, aplicando esta técnica se realizó un cribado molecular preliminar para determinar la presencia de esta mutación en una muestra de ADN de vacas Holando (H) pertenecientes a seis tambos o fincas comerciales de diferentes regiones del Uruguay. A partir de la amplificación de los productos de PCR de 170 y 146 pb se logró distinguir claramente dos genotipos: homocigota (tipo silvestre wt/wt) y heterocigota (portador de la mutación CD: MUT/wt). El genotipo homocigota wt/wt fue detectado en la muestra representativa de 103 vacas H. Se concluye que el análisis mediante PCR en tiempo real con curvas de disociación es una técnica rápida, fácilmente interpretable, de bajo costo y altamente precisa para la detección de esta mutación, el cual puede ser implementado en programas de selección genética para evitar la propagación de la enfermedad en bovinos H. ------- ABSTRACT: The purpose of this study was to optimize a real-time PCR-melting analysis for reliable and economical detection of the 7.5 Kb mutant insert of the BoERVK bovine transposable element in exon 5 of the Apolipoprotein B (APOB) gene, which causes cholesterol deficiency - CD - (OMIA 001965-9913). This technique was also used to perform a preliminary molecular screening to detect this mutation in a DNA sample of Holstein Friesian cows (HFc) of six commercial dairy farms from different regions of Uruguay. By amplifying the 170 and 146 bp PCR products, two genotypes were clearly identified: homozygote (wild type wt/wt) and heterozygote (carrier of the CD mutation: MUT/wt). The homozygous wt/wt genotype was detected in the representative sample of 103 HFc. It is concluded that Real-Time PCR-melting analysis is a fast, easily interpretable, low cost, and highly accurate technique for detecting this mutation, which can be implemented in genetic selection programs to prevent the spread of the disease in HFc. MenosRESUMEN: El objetivo de este estudio fue optimizar un análisis mediante PCR en tiempo real con curvas de disociación para la detección confiable y económica del inserto mutante de 7,5 Kb del elemento transponible bovino BoERVK en el exón 5 del gen de la Apolipoproteína B (APOB), determinante de la deficiencia de colesterol - CD - (OMIA 001965-9913). Asimismo, aplicando esta técnica se realizó un cribado molecular preliminar para determinar la presencia de esta mutación en una muestra de ADN de vacas Holando (H) pertenecientes a seis tambos o fincas comerciales de diferentes regiones del Uruguay. A partir de la amplificación de los productos de PCR de 170 y 146 pb se logró distinguir claramente dos genotipos: homocigota (tipo silvestre wt/wt) y heterocigota (portador de la mutación CD: MUT/wt). El genotipo homocigota wt/wt fue detectado en la muestra representativa de 103 vacas H. Se concluye que el análisis mediante PCR en tiempo real con curvas de disociación es una técnica rápida, fácilmente interpretable, de bajo costo y altamente precisa para la detección de esta mutación, el cual puede ser implementado en programas de selección genética para evitar la propagación de la enfermedad en bovinos H. ------- ABSTRACT: The purpose of this study was to optimize a real-time PCR-melting analysis for reliable and economical detection of the 7.5 Kb mutant insert of the BoERVK bovine transposable element in exon 5 of the Apolipoprotein B (APOB) gene, which causes cholesterol deficien... Presentar Todo |
Palabras claves : |
Cholesterol deficiency; Deficiencia de colesterol; Holstein Friesian; PCR en tiempo real con curvas de disociación; Real-time PCR-melting. |
Thesagro : |
HOLANDO. |
Asunto categoría : |
L50 Fisiología y bioquímica animal |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17399/1/38277-Texto-del-articulo-71597-2-10-20220621.pdf
|
Marc : |
LEADER 03739naa a2200277 a 4500 001 1064357 005 2023-10-24 008 2022 bl uuuu u00u1 u #d 022 $a0798-2259 (print); 2521-9715 (electronic). 024 7 $a10.52973/rcfcv-e32137$2DOI 100 1 $aBRANDA-SICA, A. 245 $aOptimización de PCR en tiempo real con curvas de disociación para la detección de la mutación causante de deficiencia de colesterol en bovinos Holando. [Optimization of Real-Time PCR-melting for detection of the Cholesterol-deficiency mutation in Holstein Friesian cattle].$h[electronic resource] 260 $c2022 500 $aArticle history: Received 15 March 2022, Accepted 30 March 2022, Published 21 June 2022. -- Correspondence author: Andrea Branda, email: abranda@inia.org.uy -- LICENSE: Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0. (https://creativecommons.org/licenses/by-nc-sa/4.0/ ). 520 $aRESUMEN: El objetivo de este estudio fue optimizar un análisis mediante PCR en tiempo real con curvas de disociación para la detección confiable y económica del inserto mutante de 7,5 Kb del elemento transponible bovino BoERVK en el exón 5 del gen de la Apolipoproteína B (APOB), determinante de la deficiencia de colesterol - CD - (OMIA 001965-9913). Asimismo, aplicando esta técnica se realizó un cribado molecular preliminar para determinar la presencia de esta mutación en una muestra de ADN de vacas Holando (H) pertenecientes a seis tambos o fincas comerciales de diferentes regiones del Uruguay. A partir de la amplificación de los productos de PCR de 170 y 146 pb se logró distinguir claramente dos genotipos: homocigota (tipo silvestre wt/wt) y heterocigota (portador de la mutación CD: MUT/wt). El genotipo homocigota wt/wt fue detectado en la muestra representativa de 103 vacas H. Se concluye que el análisis mediante PCR en tiempo real con curvas de disociación es una técnica rápida, fácilmente interpretable, de bajo costo y altamente precisa para la detección de esta mutación, el cual puede ser implementado en programas de selección genética para evitar la propagación de la enfermedad en bovinos H. ------- ABSTRACT: The purpose of this study was to optimize a real-time PCR-melting analysis for reliable and economical detection of the 7.5 Kb mutant insert of the BoERVK bovine transposable element in exon 5 of the Apolipoprotein B (APOB) gene, which causes cholesterol deficiency - CD - (OMIA 001965-9913). This technique was also used to perform a preliminary molecular screening to detect this mutation in a DNA sample of Holstein Friesian cows (HFc) of six commercial dairy farms from different regions of Uruguay. By amplifying the 170 and 146 bp PCR products, two genotypes were clearly identified: homozygote (wild type wt/wt) and heterozygote (carrier of the CD mutation: MUT/wt). The homozygous wt/wt genotype was detected in the representative sample of 103 HFc. It is concluded that Real-Time PCR-melting analysis is a fast, easily interpretable, low cost, and highly accurate technique for detecting this mutation, which can be implemented in genetic selection programs to prevent the spread of the disease in HFc. 650 $aHOLANDO 653 $aCholesterol deficiency 653 $aDeficiencia de colesterol 653 $aHolstein Friesian 653 $aPCR en tiempo real con curvas de disociación 653 $aReal-time PCR-melting 700 1 $aNICOLINI, M.P. 700 1 $aARTIGAS, R. 700 1 $aFEDERICI, M. 700 1 $aLLAMBÍ, S. 773 $tRevista Cientifica Facultad de Ciencias Veterinarias, 2022, volume 32, e32137, 1-5. https://doi.org/10.52973/rcfcv-e32137 -- OPEN ACCESS.
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